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A small nucleolar RNA functions in rRNA processing in Caenorhabditis elegans.

ABSTRACT: CeR-2 RNA is one of the newly identified Caenorhabditis elegans noncoding RNAs (ncRNAs). The characterization of CeR-2 by RNomic studies has failed to classify it into any known ncRNA family. In this study, we examined the spatiotemporal expression patterns of CeR-2 to gain insight into its function. CeR-2 is expressed in most cells from the early embryo to adult stages. The subcellular localization of this RNA is analogous to that of fibrillarin, a major protein of the nucleolus. It was observed that knockdown of C/D small nucleolar ribonucleoproteins (snoRNPs), but not of H/ACA snoRNPs, resulted in the aberrant nucleolar localization of CeR-2 RNA. A mutant worm with a reduced amount of cellular CeR-2 RNA showed changes in its pre-rRNA processing pattern compared with that of the wild-type strain N2. These results suggest that CeR-2 RNA is a C/D snoRNA involved in the processing of rRNAs.

SUBMITTER: Hokii Y 

PROVIDER: S-EPMC2943600 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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A small nucleolar RNA functions in rRNA processing in Caenorhabditis elegans.

Hokii Yusuke Y   Sasano Yumi Y   Sato Mayu M   Sakamoto Hiroshi H   Sakata Kazumi K   Shingai Ryuzo R   Taneda Akito A   Oka Shigenori S   Himeno Hyouta H   Muto Akira A   Fujiwara Toshinobu T   Ushida Chisato C  

Nucleic acids research 20100511 17

CeR-2 RNA is one of the newly identified Caenorhabditis elegans noncoding RNAs (ncRNAs). The characterization of CeR-2 by RNomic studies has failed to classify it into any known ncRNA family. In this study, we examined the spatiotemporal expression patterns of CeR-2 to gain insight into its function. CeR-2 is expressed in most cells from the early embryo to adult stages. The subcellular localization of this RNA is analogous to that of fibrillarin, a major protein of the nucleolus. It was observe  ...[more]

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