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The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism.

ABSTRACT: The crystal structure of PA1994 from Pseudomonas aeruginosa, a member of the Pfam PF06475 family classified as a domain of unknown function (DUF1089), reveals a novel fold comprising a 15-stranded ?-sheet wrapped around a single ?-helix that assembles into a tight dimeric arrangement. The remote structural similarity to lipoprotein localization factors, in addition to the presence of an acidic pocket that is conserved in DUF1089 homologs, phospholipid-binding and sugar-binding proteins, indicate a role for PA1994 and the DUF1089 family in glycolipid metabolism. Genome-context analysis lends further support to the involvement of this family of proteins in glycolipid metabolism and indicates possible activation of DUF1089 homologs under conditions of bacterial cell-wall stress or host-pathogen interactions.

SUBMITTER: Bakolitsa C 

PROVIDER: S-EPMC2954207 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism.

Bakolitsa Constantina C   Kumar Abhinav A   McMullan Daniel D   Krishna S Sri SS   Miller Mitchell D MD   Carlton Dennis D   Najmanovich Rafael R   Abdubek Polat P   Astakhova Tamara T   Chiu Hsiu Ju HJ   Clayton Thomas T   Deller Marc C MC   Duan Lian L   Elias Ylva Y   Feuerhelm Julie J   Grant Joanna C JC   Grzechnik Slawomir K SK   Han Gye Won GW   Jaroszewski Lukasz L   Jin Kevin K KK   Klock Heath E HE   Knuth Mark W MW   Kozbial Piotr P   Marciano David D   Morse Andrew T AT   Nigoghossian Edward E   Okach Linda L   Oommachen Silvya S   Paulsen Jessica J   Reyes Ron R   Rife Christopher L CL   Trout Christina V CV   van den Bedem Henry H   Weekes Dana D   White Aprilfawn A   Xu Qingping Q   Hodgson Keith O KO   Wooley John J   Elsliger Marc André MA   Deacon Ashley M AM   Godzik Adam A   Lesley Scott A SA   Wilson Ian A IA  

Acta crystallographica. Section F, Structural biology and crystallization communications 20091027 Pt 10

The crystal structure of PA1994 from Pseudomonas aeruginosa, a member of the Pfam PF06475 family classified as a domain of unknown function (DUF1089), reveals a novel fold comprising a 15-stranded β-sheet wrapped around a single α-helix that assembles into a tight dimeric arrangement. The remote structural similarity to lipoprotein localization factors, in addition to the presence of an acidic pocket that is conserved in DUF1089 homologs, phospholipid-binding and sugar-binding proteins, indicate  ...[more]

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