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ABSTRACT:
SUBMITTER: Dutoit R
PROVIDER: S-EPMC2965260 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Dutoit Raphaël R Dubois Evelyne E Jacobs Eric E
Nucleic acids research 20100811 19
Diverse tools are available for performing genetic modifications of microorganisms. However, new methods still need to be developed for performing precise genomic engineering without introducing any undesirable side-alteration. Indeed for functional analyses of genomic elements, as well as for some industrial applications, only the desired mutation should be introduced at the locus considered. This article describes a new approach fulfilling these requirements, based on the use of selection syst ...[more]