Engineering threshold-based selection systems.
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ABSTRACT: Using model organisms to identify novel therapeutic targets is frequently constrained by pre-existing genetic toolkits. To expedite positive selection for identification of novel downstream effectors, we engineered conditional expression of activated CED-10/Rac to disrupt Caenorhabditis elegans embryonic morphogenesis, titrated to 100% lethality. The strategy of engineering thresholds for positive selection using experimental animals was validated with pharmacological and genetic suppression and is generalizable to diverse molecular processes and experimental systems.
SUBMITTER: Pedone KH
PROVIDER: S-EPMC8496214 | biostudies-literature |
REPOSITORIES: biostudies-literature
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