Dataset Information
Study of the molecular recognition of aptamers selected through ovarian cancer cell-SELEX.
Ontology highlight
ABSTRACT: Background
Ovarian cancer is the most lethal gynecological malignancy, and the ovarian clear cell carcinoma subtype (OCCA) demonstrates a particularly poor response to standard treatment. Improvements in ovarian cancer outcomes, especially for OCCA, could be expected from a clearer understanding of the molecular pathology that might guide strategies for earlier diagnosis and more effective treatment.Methodology/principal findings
Cell-SELEX technology was employed to develop new molecular probes for ovarian cancer cell surface markers. A total of thirteen aptamers with K(d)'s to ovarian cancer cells in the pico- to nanomolar range were obtained. Preliminary investigation of the targets of these aptamers and their binding characteristics was also performed.Conclusions/significance
We have selected a series of aptamers that bind to different types of ovarian cancer, but not cervical cancer. Though binding to other cancer cell lines was observed, these aptamers could lead to identification of biomarkers that are related to cancer.
SUBMITTER: Van Simaeys D
PROVIDER: S-EPMC2967474 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Publications
Study of the molecular recognition of aptamers selected through ovarian cancer cell-SELEX.
PloS one 20101101 11
<h4>Background</h4>Ovarian cancer is the most lethal gynecological malignancy, and the ovarian clear cell carcinoma subtype (OCCA) demonstrates a particularly poor response to standard treatment. Improvements in ovarian cancer outcomes, especially for OCCA, could be expected from a clearer understanding of the molecular pathology that might guide strategies for earlier diagnosis and more effective treatment.<h4>Methodology/principal findings</h4>Cell-SELEX technology was employed to develop new ...[more]