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Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.

ABSTRACT: Innate immunity represents the first line of defense in animals. We report a genome-wide in vivo Drosophila RNA interference screen to uncover genes involved in susceptibility or resistance to intestinal infection with the bacterium Serratia marcescens. We first employed whole-organism gene suppression, followed by tissue-specific silencing in gut epithelium or hemocytes to identify several hundred genes involved in intestinal antibacterial immunity. Among the pathways identified, we showed that the JAK-STAT signaling pathway controls host defense in the gut by regulating stem cell proliferation and thus epithelial cell homeostasis. Therefore, we revealed multiple genes involved in antibacterial defense and the regulation of innate immunity.

SUBMITTER: Cronin SJ

PROVIDER: S-EPMC2975362 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature

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Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.

Cronin Shane J F SJ Nehme Nadine T NT Limmer Stefanie S Liegeois Samuel S Pospisilik J Andrew JA Schramek Daniel D Leibbrandt Andreas A Simoes Ricardo de Matos Rde M Gruber Susanne S Puc Urszula U Ebersberger Ingo I Zoranovic Tamara T Neely G Gregory GG von Haeseler Arndt A Ferrandon Dominique D Penninger Josef M JM

Science (New York, N.Y.) 20090611 5938

Innate immunity represents the first line of defense in animals. We report a genome-wide in vivo Drosophila RNA interference screen to uncover genes involved in susceptibility or resistance to intestinal infection with the bacterium Serratia marcescens. We first employed whole-organism gene suppression, followed by tissue-specific silencing in gut epithelium or hemocytes to identify several hundred genes involved in intestinal antibacterial immunity. Among the pathways identified, we showed that ...[more]

PMID: 19520911

Dataset Information

Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.

Publications

Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.

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