Ontology highlight
ABSTRACT:
SUBMITTER: Kubala MH
PROVIDER: S-EPMC3009406 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Kubala Marta H MH Kovtun Oleksiy O Alexandrov Kirill K Collins Brett M BM
Protein science : a publication of the Protein Society 20101201 12
The green fluorescent protein (GFP)-nanobody is a single-chain VHH antibody domain developed with specific binding activity against GFP and is emerging as a powerful tool for isolation and cellular engineering of fluorescent protein fusions in many different fields of biological research. Using X-ray crystallography and isothermal titration calorimetry, we determine the molecular details of GFP:GFP-nanobody complex formation and explain the basis of high affinity and at the same time high specif ...[more]