Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GFP tagged TRDMT1 LFQquant using GFP nanobody

ABSTRACT: In-gel digestion of GFPNb pull-out of GFP fusion TRDMT1 in damaged or undamaged HEK293 cells

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Yi Shi

PROVIDER: MSV000085313 | MassIVE | Mon Apr 20 11:56:00 BST 2020

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

GFP pulldown for the RiboVD project

Project description:GFP-tagged viral peptide sequences and their controls were used to pull down interactors from human (uninfected) or green monkey (viral infected) cell lysate. After sample preparation, digestion and bottom-up LC-MS/MS analysis, the enriched proteins were identified.

2023-03-09 | PXD033874 | Pride

iCLIP of GFP fusion proteins

Project description:iCLIP of GFP fusion proteins

| PRJEB12353 | ENA

GFP-CYYR1 interactome vs. GFP in HEK293 cells

Project description:Cell lysates from HEK293 cells individually transfected with GFP or GFP-CYYR1 were purified on GFP-Trap affinity resin and analyzed by quantitative label-free mass spectrometry. Experiments were performed in 5 biological replicates for each condition.

2024-07-25 | PXD050895 | Pride

Transcriptome and protein interaction profiling in cancer cells with mutations in histone H3.3

Project description:This entry contains proteome data extracted from co-immunoprecipitation of engineered H3F3A locus fused to GFP in HEK293 cell line, followed by PAGE separation, in-gel proteolytic digestion and LC-MS/MS identification.

2018-11-07 | PXD009966 | Pride

TLNRD1-GFP pulldown in HEK cells

Project description:In this study, we pull-down human TLNRD1-GFP and GFP from HEK cells. Binding partners were then analysed using mass-spectrometry.

2024-01-27 | PXD045258 | Pride

Exploration of GFP-IRF7 neddylation sites

Project description:To explore the possible neddylation sites, GFP-IRF7 and His-NEDD8 were co-overexpressed in HEK293 cell line and GFP was immunoprecipitated, followed by SDS-PAGE and silver staining. The band detected was subjected to LC–MS/MS.

2021-08-19 | PXD024052 | Pride

RNAseq of GFP-sorted cells (Low-GFP vs. High-GFP, GFP-fed vs GFP+fed)

Project description:RNAseq of GFP-sorted cells (Low-GFP vs. High-GFP, GFP-fed vs GFP+fed)

| PRJNA544322 | ENA

Talin1-GFP pulldown in U2OS cells

Project description:In this study, we pull-down human Talin1-GFP and GFP from U2OS cells plated on fibronectin. Binding partners were then analysed using mass-spectrometry.

2021-05-12 | PXD024634 | Pride

IP-MS of GFP tagged MRV μ2 protein

Project description:To determine which cellular proteins interacts with the viral MRV protein μ2 and its mutant μ2-P208S, GFP-tagged constructions of μ2 and μ2-P208S in both C- and N- terminus were transfected in 293T cells alongside control GFP alone for 24h, and submitted to GFP pulldown before IP were prepared for MS analysis.

2022-06-09 | PXD029701 | Pride

TLA: Targeted Locus Amplification interaction libraries from TLA viewpoints in the GFP construct from GFP(CAG) cells.

Project description:We performed Targeted Locus Amplification (TLA) to map the insertion site of a stably integrated, hemizygous ectopic locus that contains CAG repeats (15 or 270 repeats) in clonal populations of HEK293-derived cells (GFP(CAG)15 or GFP(CAG)270).

2020-04-07 | GSE148183 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data