Dynamic histone acetylation is critical for cotranscriptional spliceosome assembly and spliceosomal rearrangements.

ABSTRACT: Assembly of the spliceosome onto pre-mRNA is a dynamic process involving the ordered exchange of snRNPs to form the catalytically active spliceosome. These ordered rearrangements have recently been shown to occur cotranscriptionally, while the RNA polymerase is still actively engaged with the chromatin template. We previously demonstrated that the histone acetyltransferase Gcn5 is required for U2 snRNP association with the branchpoint. Here we provide evidence that histone acetylation and deacetylation facilitate proper cotranscriptional association of spliceosomal snRNPs. As with GCN5, mutation or deletion of Gcn5-targeted histone H3 residues leads to synthetic lethality when combined with deletion of the genes encoding the U2 snRNP components Lea1 or Msl1. Gcn5 associates throughout intr

SUBMITTER: Gunderson FQ 

PROVIDER: S-EPMC3033250 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature