Ontology highlight
ABSTRACT:
SUBMITTER: Jennings-Antipov LD
PROVIDER: S-EPMC3033316 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Jennings-Antipov Laura D LD Song Likai L Collier R John RJ
Proceedings of the National Academy of Sciences of the United States of America 20110124 5
The protective antigen (PA) moiety of anthrax toxin forms oligomeric pores that translocate the enzymatic moieties of the toxin--lethal factor (LF) and edema factor (EF)--across the endosomal membrane of mammalian cells. Here we describe site-directed spin-labeling studies that identify interactions of LF with the prepore and pore conformations of PA. Our results reveal a direct interaction between the extreme N terminus of LF (residues 2-5) and the Φ-clamp, a structure within the lumen of the p ...[more]