Ontology highlight
ABSTRACT:
SUBMITTER: O'Shaughnessy EC
PROVIDER: S-EPMC3035479 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
O'Shaughnessy Ellen C EC Palani Santhosh S Collins James J JJ Sarkar Casim A CA
Cell 20110101 1
The flexibility of MAPK cascade responses enables regulation of a vast array of cell fate decisions, but elucidating the mechanisms underlying this plasticity is difficult in endogenous signaling networks. We constructed insulated mammalian MAPK cascades in yeast to explore how intrinsic and extrinsic perturbations affect the flexibility of these synthetic signaling modules. Contrary to biphasic dependence on scaffold concentration, we observe monotonic decreases in signal strength as scaffold c ...[more]