Microsecond single-molecule tracking (?sSMT).
Ontology highlight
ABSTRACT:
SUBMITTER: Semrau S
PROVIDER: S-EPMC3037721 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Publications
Microsecond single-molecule tracking (μsSMT).
Semrau Stefan S Pezzarossa Anna A Schmidt Thomas T
Biophysical journal 20110201 4
Here we report on a method to track individual molecules on nanometer length and microsecond timescales using an optical microscope. Our method is based on double-labeling of a molecule with two spectrally distinct fluorophores and illuminating it with laser pulses of different wavelengths that partially overlap temporally. We demonstrate our method by using it to resolve the motion of short DNA oligomers in solution down to a timescale of 100 μs. ...[more]
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