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Genomic sequencing and analysis of a Chinese hamster ovary cell line using Illumina sequencing technology.

ABSTRACT: BACKGROUND: Chinese hamster ovary (CHO) cells are among the most widely used hosts for therapeutic protein production. Yet few genomic resources are available to aid in engineering high-producing cell lines. RESULTS: High-throughput Illumina sequencing was used to generate a 1x genomic coverage of an engineered CHO cell line expressing secreted alkaline phosphatase (SEAP). Reference-guided alignment and assembly produced 3.57 million contigs and CHO-specific sequence information for ~ 18,000 mouse and ~ 19,000 rat orthologous genes. The majority of these genes are involved in metabolic processes, cellular signaling, and transport and represent attractive targets for cell line engineering. CONCLUSIONS: This demonstrates the applicability of next-generation sequencing technology and comparative genomic analysis in the development of CHO genomic resources.

SUBMITTER: Hammond S

PROVIDER: S-EPMC3038171 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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Genomic sequencing and analysis of a Chinese hamster ovary cell line using Illumina sequencing technology.

Hammond Stephanie S Swanberg Jeffrey C JC Kaplarevic Mihailo M Lee Kelvin H KH

BMC genomics 20110126

<h4>Background</h4>Chinese hamster ovary (CHO) cells are among the most widely used hosts for therapeutic protein production. Yet few genomic resources are available to aid in engineering high-producing cell lines.<h4>Results</h4>High-throughput Illumina sequencing was used to generate a 1x genomic coverage of an engineered CHO cell line expressing secreted alkaline phosphatase (SEAP). Reference-guided alignment and assembly produced 3.57 million contigs and CHO-specific sequence information for ...[more]

PMID: 21269493

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