Ontology highlight
ABSTRACT:
SUBMITTER: Xu X
PROVIDER: S-EPMC3164356 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Xu Xun X Nagarajan Harish H Lewis Nathan E NE Pan Shengkai S Cai Zhiming Z Liu Xin X Chen Wenbin W Xie Min M Wang Wenliang W Hammond Stephanie S Andersen Mikael R MR Neff Norma N Passarelli Benedetto B Koh Winston W Fan H Christina HC Wang Jianbin J Gui Yaoting Y Lee Kelvin H KH Betenbaugh Michael J MJ Quake Stephen R SR Famili Iman I Palsson Bernhard O BO Wang Jun J
Nature biotechnology 20110731 8
Chinese hamster ovary (CHO)-derived cell lines are the preferred host cells for the production of therapeutic proteins. Here we present a draft genomic sequence of the CHO-K1 ancestral cell line. The assembly comprises 2.45 Gb of genomic sequence, with 24,383 predicted genes. We associate most of the assembled scaffolds with 21 chromosomes isolated by microfluidics to identify chromosomal locations of genes. Furthermore, we investigate genes involved in glycosylation, which affect therapeutic pr ...[more]