Ontology highlight
ABSTRACT:
SUBMITTER: Braun CR
PROVIDER: S-EPMC3048092 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Chemistry & biology 20101201 12
Defining protein interactions forms the basis for discovery of biological pathways, disease mechanisms, and opportunities for therapeutic intervention. To harness the robust binding affinity and selectivity of structured peptides for interactome discovery, we engineered photoreactive stapled BH3 peptide helices that covalently capture their physiologic BCL-2 family targets. The crosslinking α helices covalently trap both static and dynamic protein interactors, and enable rapid identification of ...[more]