Ontology highlight
ABSTRACT:
SUBMITTER: Lam BJ
PROVIDER: S-EPMC3048896 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Lam Bianca J BJ Joyce Gerald F GF
Journal of the American Chemical Society 20110215 9
A system was devised that enables quantitative, ligand-dependent exponential amplification for various ligands that can be recognized by an RNA aptamer. The aptamer is linked to an RNA enzyme that catalyzes the joining of two oligonucleotide substrates. The product of this reaction is another RNA enzyme that undergoes self-sustained replication at constant temperature, increasing in copy number exponentially. The concentration of the ligand determines the amount of time required for the replicat ...[more]