Ontology highlight
ABSTRACT:
SUBMITTER: Bruderer R
PROVIDER: S-EPMC3049431 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Bruderer Roland R Tatham Michael H MH Plechanovova Anna A Matic Ivan I Garg Amit K AK Hay Ronald T RT
EMBO reports 20110121 2
The small ubiquitin-like modifier (SUMO) can undergo self-modification to form polymeric chains that have been implicated in cellular processes such as meiosis, genome maintenance and stress response. Investigations into the biological role of polymeric chains have been hampered by the absence of a protocol for the purification of proteins linked to SUMO chains. In this paper, we describe a rapid affinity purification procedure for the isolation of endogenous polySUMO-modified species that gener ...[more]