Ontology highlight
ABSTRACT:
SUBMITTER: Pan Y
PROVIDER: S-EPMC3052315 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Pan Yuqiong Y Ouyang Zhengqing Z Wong Wing Hung WH Baker Julie C JC
PloS one 20110309 3
We show that high quality microarray gene expression profiles can be obtained following FACS sorting of cells using combinations of transcription factors. We use this transcription factor FACS (tfFACS) methodology to perform a genomic analysis of hESC-derived endodermal lineages marked by combinations of SOX17, GATA4, and CXCR4, and find that triple positive cells have a much stronger definitive endoderm signature than other combinations of these markers. Additionally, SOX17(+) GATA4(+) cells ca ...[more]