Ontology highlight
ABSTRACT:
SUBMITTER: Ghosh-Kumar M
PROVIDER: S-EPMC3074133 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Ghosh-Kumar Manjira M Alam Tanfis I TI Draper Bonnie B Stack John D JD Rao Venigalla B VB
Nucleic acids research 20101124 7
In genome packaging by tailed bacteriophages and herpesviruses, a concatemeric DNA is cut and inserted into an empty procapsid. A series of cuts follow the encapsidation of each unit-length 'headful' genome, but the mechanisms by which cutting is coupled to packaging are not understood. Here we report the first biochemical characterization of a headful nuclease from bacteriophage T4. Our results show that the T4 nuclease, which resides in the C-terminal domain of large 'terminase' gp17, is a wea ...[more]