Ontology highlight
ABSTRACT:
SUBMITTER: Kulevich SE
PROVIDER: S-EPMC3075559 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Kulevich Suzanne E SE Frey Brian L BL Kreitinger Gloria G Smith Lloyd M LM
Analytical chemistry 20101129 24
A major limitation of mass spectrometry-based proteomics is inefficient and differential ionization during electrospray ionization (ESI). This leads to problems such as increased limits of detection and incomplete sequence coverage of proteins. Incomplete sequence coverage is especially problematic for analyses that require the detection and identification of specific peptides from a protein, such as the analysis of post-translational modifications. We describe here the development and use of al ...[more]