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Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing.

ABSTRACT: In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio and field of view or to image different axial planes simultaneously. Using spatiotemporal multiplexing we circumvented light-scattering ambiguity inherent to deep-tissue multifocal two-photon microscopy. We demonstrate calcium imaging at multiple axial planes in the intact mouse brain to monitor network activity of ensembles of cortical neurons in three spatial dimensions.

SUBMITTER: Cheng A

PROVIDER: S-EPMC3076599 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

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Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing.

Cheng Adrian A Gonçalves J Tiago JT Golshani Peyman P Arisaka Katsushi K Portera-Cailliau Carlos C

Nature methods 20110109 2

In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio and field of view or to image different axial planes simultaneously. Using spatiotemporal multiplexing we circumvented light-scattering ambiguity inherent to deep-tissue multifocal two-photon microscopy. We demonstrate calcium imaging at multiple axial planes in the intact mouse brain to monitor network activity of ensembles of cortical neurons in three spa ...[more]

PMID: 21217749

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