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Simultaneous multiplane imaging with reverberation two-photon microscopy.

ABSTRACT: Multiphoton microscopy has gained enormous popularity because of its unique capacity to provide high-resolution images from deep within scattering tissue. Here, we demonstrate video-rate multiplane imaging with two-photon microscopy by performing near-instantaneous axial scanning while maintaining three-dimensional micrometer-scale resolution. Our technique, termed reverberation microscopy, enables the monitoring of neuronal populations over large depth ranges and can be implemented as a simple add-on to a conventional design.

SUBMITTER: Beaulieu DR

PROVIDER: S-EPMC8590882 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Simultaneous multiplane imaging with reverberation two-photon microscopy.

Beaulieu Devin R DR Davison Ian G IG Kılıç Kıvılcım K Bifano Thomas G TG Mertz Jerome J

Nature methods 20200210 3

Multiphoton microscopy has gained enormous popularity because of its unique capacity to provide high-resolution images from deep within scattering tissue. Here, we demonstrate video-rate multiplane imaging with two-photon microscopy by performing near-instantaneous axial scanning while maintaining three-dimensional micrometer-scale resolution. Our technique, termed reverberation microscopy, enables the monitoring of neuronal populations over large depth ranges and can be implemented as a simple ...[more]

PMID: 32042186

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