Blockade of class IB phosphoinositide-3 kinase ameliorates obesity-induced inflammation and insulin resistance.

ABSTRACT: Obesity and insulin resistance, the key features of metabolic syndrome, are closely associated with a state of chronic, low-grade inflammation characterized by abnormal macrophage infiltration into adipose tissues. Although it has been reported that chemokines promote leukocyte migration by activating class IB phosphoinositide-3 kinase (PI3K?) in inflammatory states, little is known about the role of PI3K? in obesity-induced macrophage infiltration into tissues, systemic inflammation, and the development of insulin resistance. In the present study, we used murine models of both diet-induced and genetically induced obesity to examine the role of PI3K? in the accumulation of tissue macrophages and the development of obesity-induced insulin resistance. Mice lacking p110? (Pik3cg(-/-)), the catalytic subunit of PI3K?, exhibited improved systemic insulin sensitivity with enhanced insulin signaling in the tissues of obese animals. In adipose tissues and livers of obese Pik3cg(-/-) mice, the numbers of infiltrated proinflammatory macrophages were markedly reduced, leading to suppression of inflammatory reactions in these tissues. Furthermore, bone marrow-specific deletion and pharmacological blockade of PI3K? also ameliorated obesity-induced macrophage infiltration and insulin resistance. These data suggest that PI3K? plays a crucial role in the development of both obesity-induced inflammation and systemic insulin resistance and that PI3K? can be a therapeutic target for type 2 diabetes.

SUBMITTER: Kobayashi N 

PROVIDER: S-EPMC3078379 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature