Breaking up the C complex spliceosome shows stable association of proteins with the lariat intron intermediate.

ABSTRACT: Spliceosome assembly requires several structural rearrangements to position the components of the catalytic core. Many of these rearrangements involve successive strengthening and weakening of different RNA:RNA and RNA:proteins interactions within the complex. To gain insight into the organization of the catalytic core of the spliceosome arrested between the two steps of splicing chemistry (C complex), we investigated the effects of exposing C complex to low concentrations of urea. We find that in the presence of 3M urea C complex separates into at least three sub-complexes. One sub-complex contains the 5'exon, another contains the intron-lariat intermediate, and U2/U5/U6 snRNAs likely comprise a third sub-complex. We purified the intron-lariat intermediate sub-complex and identified sever

SUBMITTER: Coltri P 

PROVIDER: S-EPMC3079748 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature