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Preliminary X-ray crystallographic analysis of the glycosyltransferase from a marine Streptomyces species.

ABSTRACT: ElaGT is a glycosyltransferase from a marine Streptomyces species that is involved in the biosynthesis of elaiophylin. Here, the molecular cloning, protein expression and purification, preliminary crystallization and crystallographic characterization of ElaGT are reported. The rod-shaped crystals belonged to space group P2(1)22, with unit-cell parameters a=66.7, b=131.7, c=224.6?Å, ?=90, ?=90, ?=90°. Data were collected to 2.9?Å resolution. A preliminary molecular-replacement solution implied the presence of two ElaGT molecules in the asymmetric unit.

SUBMITTER: Gong L

PROVIDER: S-EPMC3079993 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

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Preliminary X-ray crystallographic analysis of the glycosyltransferase from a marine Streptomyces species.

Gong Liping L Xiao Yi Y Liu Qiang Q Li Sumei S Zhang Changsheng C Liu Jinsong J

Acta crystallographica. Section F, Structural biology and crystallization communications 20101223 Pt 1

ElaGT is a glycosyltransferase from a marine Streptomyces species that is involved in the biosynthesis of elaiophylin. Here, the molecular cloning, protein expression and purification, preliminary crystallization and crystallographic characterization of ElaGT are reported. The rod-shaped crystals belonged to space group P2(1)22, with unit-cell parameters a=66.7, b=131.7, c=224.6 Å, α=90, β=90, γ=90°. Data were collected to 2.9 Å resolution. A preliminary molecular-replacement solution implied th ...[more]

PMID: 21206045

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