A parallel microfluidic flow cytometer for high-content screening.
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ABSTRACT:
SUBMITTER: McKenna BK
PROVIDER: S-EPMC3084896 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Publications
A parallel microfluidic flow cytometer for high-content screening.
McKenna Brian K BK Evans James G JG Cheung Man Ching MC Ehrlich Daniel J DJ
Nature methods 20110410 5
A parallel microfluidic cytometer (PMC) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count, one-dimensional imaging with flow cytometry. The 384 parallel flow channels of the PMC decouple count rate from signal-to-noise ratio. Using six-pixel one-dimensional images, we investigated protein localization in a yeast model for human protein misfolding diseases and demonstrated the feasibility of a nuclear-translocation assay in Chinese hamster ovary (CHO) cells expr ...[more]
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