Ontology highlight
ABSTRACT:
SUBMITTER: Nash RP
PROVIDER: S-EPMC3086049 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
FEBS letters 20110323 8
Conjugative plasmid transfer results in the spread of antibiotic resistance genes and virulence factors between bacterial cells. Plasmid transfer is dependent upon the DNA nicking activity of a plasmid-encoded relaxase enzyme. Tyrosine residues within the relaxase cleave the DNA plasmid nic site in a highly sequence-specific manner. The conjugative resistance plasmid pCU1 encodes a relaxase with four tyrosine residues surrounding its active site (Y18,19,26,27). We use activity assays to demonstr ...[more]