Ontology highlight
ABSTRACT:
SUBMITTER: Okazaki IM
PROVIDER: S-EPMC3093487 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Okazaki Il-mi IM Okawa Katsuya K Kobayashi Maki M Yoshikawa Kiyotsugu K Kawamoto Shimpei S Nagaoka Hitoshi H Shinkura Reiko R Kitawaki Yoko Y Taniguchi Hisaaki H Natsume Tohru T Iemura Shun-Ichiro S Honjo Tasuku T
Proceedings of the National Academy of Sciences of the United States of America 20110425 19
Activation-induced cytidine deaminase (AID) is shown to be essential and sufficient to induce two genetic alterations in the Ig loci: class switch recombination (CSR) and somatic hypermutation (SHM). However, it is still unknown how a single-molecule AID differentially regulates CSR and SHM. Here we identified Spt6 as an AID-interacting protein by yeast two-hybrid screening and immunoprecipitation followed by mass spectrometry. Knockdown of Spt6 resulted in severe reduction of CSR in both the en ...[more]