Context-dependent function of "GATA switch" sites in vivo.
Ontology highlight
ABSTRACT:
SUBMITTER: Snow JW
PROVIDER: S-EPMC3100688 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Publications
Context-dependent function of "GATA switch" sites in vivo.
Snow Jonathan W JW Trowbridge Jennifer J JJ Johnson Kirby D KD Fujiwara Tohru T Emambokus Nikla E NE Grass Jeffrey A JA Orkin Stuart H SH Bresnick Emery H EH
Blood 20110311 18
Master transcriptional regulators of development often function through dispersed cis elements at endogenous target genes. While cis-elements are routinely studied in transfection and transgenic reporter assays, it is challenging to ascertain how they function in vivo. To address this problem in the context of the locus encoding the critical hematopoietic transcription factor Gata2, we engineered mice lacking a cluster of GATA motifs 2.8 kb upstream of the Gata2 transcriptional start site. We de ...[more]
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