Ontology highlight
ABSTRACT:
SUBMITTER: Popchock AR
PROVIDER: S-EPMC5216134 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Popchock Andrew R AR Tseng Kuo-Fu KF Wang Pan P Karplus P Andrew PA Xiang Xin X Qiu Weihong W
Nature communications 20170104
Kinesin-14s are commonly known as nonprocessive minus end-directed microtubule motors that function mainly for mitotic spindle assembly. Here we show using total internal reflection fluorescence microscopy that KlpA-a kinesin-14 from Aspergillus nidulans-is a context-dependent bidirectional motor. KlpA exhibits plus end-directed processive motility on single microtubules, but reverts to canonical minus end-directed motility when anchored on the surface in microtubule-gliding experiments or inter ...[more]