Ontology highlight
ABSTRACT:
SUBMITTER: Metz S
PROVIDER: S-EPMC3100918 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Metz Silke S Haas Alexander K AK Daub Karin K Croasdale Rebecca R Stracke Jan J Lau Wilma W Georges Guy G Josel Hans-Peter HP Dziadek Sebastian S Hopfner Karl-Peter KP Lammens Alfred A Scheuer Werner W Hoffmann Eike E Mundigl Olaf O Brinkmann Ulrich U
Proceedings of the National Academy of Sciences of the United States of America 20110502 20
Bispecific antibodies that bind cell-surface targets as well as digoxigenin (Dig) were generated for targeted payload delivery. Targeting moieties are IgGs that bind the tumor antigens Her2, IGF1R, CD22, or LeY. A Dig-binding single-chain Fv was attached in disulfide-stabilized form to C termini of CH3 domains of targeting antibodies. Bispecific molecules were expressed in mammalian cells and purified in the same manner as unmodified IgGs. They are stable without aggregation propensity and retai ...[more]