Ontology highlight
ABSTRACT:
SUBMITTER: Evans BS
PROVIDER: S-EPMC3102229 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Evans Bradley S BS Chen Yunqiu Y Metcalf William W WW Zhao Huimin H Kelleher Neil L NL
Chemistry & biology 20110501 5
Many lead compounds in the search for new drugs derive from peptides and polyketides whose similar biosynthetic enzymes have been difficult to engineer for production of new derivatives. Problems with generating multiple analogs in a single experiment along with lack of high-throughput methods for structure-based screening have slowed progress in this area. Here, we use directed evolution and a multiplexed assay to screen a library of >14,000 members to generate three derivatives of the antibact ...[more]