Ontology highlight
ABSTRACT:
SUBMITTER: Kloiber K
PROVIDER: S-EPMC3105391 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Kloiber Karin K Spitzer Romana R Tollinger Martin M Konrat Robert R Kreutz Christoph C
Nucleic acids research 20110119 10
The refolding kinetics of bistable RNA sequences were studied in unperturbed equilibrium via (13)C exchange NMR spectroscopy. For this purpose a straightforward labeling technique was elaborated using a 2'-(13)C-methoxy uridine modification, which was prepared by a two-step synthesis and introduced into RNA using standard protocols. Using (13)C longitudinal exchange NMR spectroscopy the refolding kinetics of a 20 nt bistable RNA were characterized at temperatures between 298 and 310K, yielding t ...[more]