Unknown

Dataset Information

0

Analyses of subnanometer resolution cryo-EM density maps.


ABSTRACT: Today, electron cryomicroscopy (cryo-EM) can routinely achieve subnanometer resolutions of complex macromolecular assemblies. From a density map, one can extract key structural and functional information using a variety of computational analysis tools. At subnanometer resolution, these tools make it possible to isolate individual subunits, identify secondary structures, and accurately fit atomic models. With several cryo-EM studies achieving resolutions beyond 5Å, computational modeling and feature recognition tools have been employed to construct backbone and atomic models of the protein components directly from a density map. In this chapter, we describe several common classes of computational tools that can be used to analyze and model subnanometer resolution reconstructions from cryo-EM. A general protocol for analyzing subnanometer resolution density maps is presented along with a full description of steps used in analyzing the 4.3Å resolution structure of Mm-cpn.

SUBMITTER: Baker ML 

PROVIDER: S-EPMC3107677 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature

altmetric image

Publications

Analyses of subnanometer resolution cryo-EM density maps.

Baker Matthew L ML   Baker Mariah R MR   Hryc Corey F CF   Dimaio Frank F  

Methods in enzymology 20100101


Today, electron cryomicroscopy (cryo-EM) can routinely achieve subnanometer resolutions of complex macromolecular assemblies. From a density map, one can extract key structural and functional information using a variety of computational analysis tools. At subnanometer resolution, these tools make it possible to isolate individual subunits, identify secondary structures, and accurately fit atomic models. With several cryo-EM studies achieving resolutions beyond 5Å, computational modeling and feat  ...[more]

Similar Datasets

| S-EPMC9645795 | biostudies-literature
| S-EPMC3903095 | biostudies-literature
| S-EPMC8294179 | biostudies-literature
| S-EPMC7543659 | biostudies-literature
| S-EPMC6999609 | biostudies-literature
| S-EPMC7063051 | biostudies-literature
| S-EPMC8025875 | biostudies-literature
| S-EPMC7484085 | biostudies-literature
| S-EPMC5679758 | biostudies-literature
| S-EPMC3899894 | biostudies-literature