Quantifying the local resolution of cryo-EM density maps.
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ABSTRACT:
SUBMITTER: Kucukelbir A
PROVIDER: S-EPMC3903095 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Publications
Quantifying the local resolution of cryo-EM density maps.
Kucukelbir Alp A Sigworth Fred J FJ Tagare Hemant D HD
Nature methods 20131110 1
We propose a definition of local resolution for three-dimensional electron cryo-microscopy (cryo-EM) density maps that uses local sinusoidal features. Our algorithm has no free parameters and is applicable to other imaging modalities, including tomography. By evaluating the local resolution of single-particle reconstructions and subtomogram averages for four example data sets, we report variable resolution across a 4- to 40-Å range. ...[more]
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