Ontology highlight
ABSTRACT:
SUBMITTER: Terwilliger TC
PROVIDER: S-EPMC7484085 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Terwilliger Thomas C TC Ludtke Steven J SJ Read Randy J RJ Adams Paul D PD Afonine Pavel V PV
Nature methods 20200817 9
A density-modification procedure for improving maps from single-particle electron cryogenic microscopy (cryo-EM) is presented. The theoretical basis of the method is identical to that of maximum-likelihood density modification, previously used to improve maps from macromolecular X-ray crystallography. Key differences from applications in crystallography are that the errors in Fourier coefficients are largely in the phases in crystallography but in both phases and amplitudes in cryo-EM, and that ...[more]