Ontology highlight
ABSTRACT:
SUBMITTER: Aubin-Tam ME
PROVIDER: S-EPMC3108460 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Aubin-Tam Marie-Eve ME Olivares Adrian O AO Sauer Robert T RT Baker Tania A TA Lang Matthew J MJ
Cell 20110401 2
All cells employ ATP-powered proteases for protein-quality control and regulation. In the ClpXP protease, ClpX is a AAA+ machine that recognizes specific protein substrates, unfolds these molecules, and then translocates the denatured polypeptide through a central pore and into ClpP for degradation. Here, we use optical-trapping nanometry to probe the mechanics of enzymatic unfolding and translocation of single molecules of a multidomain substrate. Our experiments demonstrate the capacity of Clp ...[more]