Ontology highlight
ABSTRACT:
SUBMITTER: Baytshtok V
PROVIDER: S-EPMC5392565 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Baytshtok Vladimir V Chen Jiejin J Glynn Steven E SE Nager Andrew R AR Grant Robert A RA Baker Tania A TA Sauer Robert T RT
The Journal of biological chemistry 20170221 14
The HslUV proteolytic machine consists of HslV, a double-ring self-compartmentalized peptidase, and one or two AAA+ HslU ring hexamers that hydrolyze ATP to power the unfolding of protein substrates and their translocation into the proteolytic chamber of HslV. Here, we use genetic tethering and disulfide bonding strategies to construct HslU pseudohexamers containing mixtures of ATPase active and inactive subunits at defined positions in the hexameric ring. Genetic tethering impairs HslV binding ...[more]