Dataset Information

Dynamic expression patterns of imprinted genes in human embryonic stem cells following prolonged passaging and differentiation.

ABSTRACT:

Purpose

To evaluate the overall expression patterns of imprinted genes in human embryonic stem cells following long term culture and differentiation.

Materials and methods

Expression levels of 65 imprinted genes determined by PCR array were analyzed in one human embryonic stem cell line (cHES1) following prolonged passaging and differentiation.

Results

Transcripts of 63 imprinted genes were detected in cHES1 cells. Expression levels of all but 5 imprinted genes did not correlate with passage numbers or differ in cells after passage 50 compared with those before passage 50. SLC22A2, SLC22A3, CPA, H19, COPG2IT1 and IGF2 expression were significantly increased in embryoid bodies compared with undifferentiated cells.

Conclusions

The global expression profiles of imprinted genes are generally stable in human embryonic stem cells after prolonged passaging and differentiation.

SUBMITTER: Mai X

PROVIDER: S-EPMC3114963 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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Publications

Dynamic expression patterns of imprinted genes in human embryonic stem cells following prolonged passaging and differentiation.

Mai Xiuyun X Mai Qingyun Q Li Tao T Zhou Canquan C

Journal of assisted reproduction and genetics 20101216 4

<h4>Purpose</h4>To evaluate the overall expression patterns of imprinted genes in human embryonic stem cells following long term culture and differentiation.<h4>Materials and methods</h4>Expression levels of 65 imprinted genes determined by PCR array were analyzed in one human embryonic stem cell line (cHES1) following prolonged passaging and differentiation.<h4>Results</h4>Transcripts of 63 imprinted genes were detected in cHES1 cells. Expression levels of all but 5 imprinted genes did not corr ...[more]

PMID: 21161363

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Project description:BackgroundThe unique features of human embryonic stem (hES) cells make them the best candidate resource for both cell replacement therapy and development research. However, the molecular mechanisms responsible for the simultaneous maintenance of their self-renewal properties and undifferentiated state remain unclear. Non-coding microRNAs (miRNA) which regulate mRNA cleavage and inhibit encoded protein translation exhibit temporal or tissue-specific expression patterns and they play an important role in development timing.ResultsIn this study, we analyzed miRNA and gene expression profiles among samples from 3 hES cell lines (H9, I6 and BG01v), differentiated embryoid bodies (EB) derived from H9 cells at different time points, and 5 adult cell types including Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal Human Astrocytes (NHA), and Lung Fibroblasts (LFB). This analysis rendered 104 miRNAs and 776 genes differentially expressed among the three cell types. Selected differentially expressed miRNAs and genes were further validated and confirmed by quantitative real-time-PCR (qRT-PCR). Especially, members of the miR-302 cluster on chromosome 4 and miR-520 cluster on chromosome 19 were highly expressed in undifferentiated hES cells. MiRNAs in these two clusters displayed similar expression levels. The members of these two clusters share a consensus 7-mer seed sequence and their targeted genes had overlapping functions. Among the targeted genes, genes with chromatin structure modification function are enriched suggesting a role in the maintenance of chromatin structure. We also found that the expression level of members of the two clusters, miR-520b and miR-302c, were negatively correlated with their targeted genes based on gene expression analysisConclusionWe identified the expression patterns of miRNAs and gene transcripts in the undifferentiation of human embryonic stem cells; among the miRNAs that are highly expressed in undifferentiated embryonic stem cells, the miR-520 cluster may be closely involved in hES cell function and its relevance to chromatin structure warrants further study.

Dataset Information

Dynamic expression patterns of imprinted genes in human embryonic stem cells following prolonged passaging and differentiation.

Purpose

Materials and methods

Results

Conclusions

Publications

Dynamic expression patterns of imprinted genes in human embryonic stem cells following prolonged passaging and differentiation.

Similar Datasets

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