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Perturbation of cytoskeleton dynamics by the opposing effects of Rac1 and Rac1b.

ABSTRACT: Rac1, a ubiquitously expressed member of the Rho GTPase family, plays a pivotal role in the regulation of multiple cellular processes including cytoskeleton reorganization, cell growth, differentiation and motility. Here we show that the tumor-specific splice variant of Rac1, Rac1b, negatively regulates Rac1 activity. The expression of Rac1b in HeLa cells interferes with Rac1 activation by PDGF, leads to a reduction in membrane-bound Rac1 and promotes an increase in Rho activity. The antagonistic relationship between Rac1 and Rac1b perturbs the regulatory circuitry that controls actin cytoskeleton dynamics thereby leading to tumor-linked alterations in cell morphology and motility.

SUBMITTER: Nimnual AS

PROVIDER: S-EPMC3116599 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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Perturbation of cytoskeleton dynamics by the opposing effects of Rac1 and Rac1b.

Nimnual Anjaruwee S AS Taylor Laura J LJ Nyako Marian M Jeng Hao-Hsuan HH Bar-Sagi Dafna D

Small GTPases 20100901 2

Rac1, a ubiquitously expressed member of the Rho GTPase family, plays a pivotal role in the regulation of multiple cellular processes including cytoskeleton reorganization, cell growth, differentiation and motility. Here we show that the tumor-specific splice variant of Rac1, Rac1b, negatively regulates Rac1 activity. The expression of Rac1b in HeLa cells interferes with Rac1 activation by PDGF, leads to a reduction in membrane-bound Rac1 and promotes an increase in Rho activity. The antagonisti ...[more]

PMID: 21686260

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Project description:A sustained dynamic inflow perturbation and bar-floodplain conversion are considered crucial to dynamic meandering. Past experiments, one-dimensional modelling and linear theory have demonstrated that the initiation and persistence of dynamic meandering require a periodic transverse motion of the inflow. However, it remains unknown whether the period of the inflow perturbation affects self-formed meander dynamics. Here, we numerically study the effect of the inflow perturbation period on the development and meander dynamics of a chute-cutoff-dominated river, which requires two-dimensional modelling with vegetation forming floodplain on bars. We extended the morphodynamic model Nays2D with growth and mortality rules of vegetation to allow for meandering. We tested the effect of a transversely migrating inflow boundary by varying the perturbation period between runs over an order of magnitude around typical modelled meander periods. Following the cutoff cascade after initial meander formation from a straight channel, all runs with sufficient vegetation show series of growing meanders terminated by chute cutoffs. This generates an intricate channel belt topography with point bar complexes truncated by chutes, oxbow lakes, and scroll-bar-related vegetation age patterns. The sinuosity, braiding index and meander period, which emerge from the inherent biomorphological feedback loops, are unrelated to the inflow perturbation period, although the spin-up to dynamic equilibrium takes a longer time and distance for weak and absent inflow perturbations. This explains why, in previous experimental studies, dynamic meandering was only accomplished with a sustained upstream perturbation in flumes that were short relative to the meander wavelength. Our modelling of self-formed meander patterns is evidence that scroll-bar-dominated and chute-cutoff-dominated meanders develop from downstream convecting instabilities. This insight extends to many more fluvial, estuarine and coastal systems in morphological models and experiments, which require sustained dynamic perturbations to form complex patterns and develop natural dynamics. © 2019 The Authors. Earth Surface Processes and Landforms Published by John Wiley & Sons Ltd.

Dataset Information

Perturbation of cytoskeleton dynamics by the opposing effects of Rac1 and Rac1b.

Publications

Perturbation of cytoskeleton dynamics by the opposing effects of Rac1 and Rac1b.

Similar Datasets

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