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Vernalization-repression of Arabidopsis FLC requires promoter sequences but not antisense transcripts.

ABSTRACT: The repression of Arabidopsis FLC expression by vernalization (extended cold) has become a model for understanding polycomb-associated epigenetic regulation in plants. Antisense and sense non-coding RNAs have been respectively implicated in initiation and maintenance of FLC repression by vernalization. We show that the promoter and first exon of the FLC gene are sufficient to initiate repression during vernalization; this initial repression of FLC does not require antisense transcription. Long-term maintenance of FLC repression requires additional regions of the gene body, including those encoding sense non-coding transcripts.

SUBMITTER: Helliwell CA

PROVIDER: S-EPMC3119698 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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Vernalization-repression of Arabidopsis FLC requires promoter sequences but not antisense transcripts.

Helliwell Chris A CA Robertson Masumi M Finnegan E Jean EJ Buzas Diana M DM Dennis Elizabeth S ES

PloS one 20110621 6

The repression of Arabidopsis FLC expression by vernalization (extended cold) has become a model for understanding polycomb-associated epigenetic regulation in plants. Antisense and sense non-coding RNAs have been respectively implicated in initiation and maintenance of FLC repression by vernalization. We show that the promoter and first exon of the FLC gene are sufficient to initiate repression during vernalization; this initial repression of FLC does not require antisense transcription. Long-t ...[more]

PMID: 21713009

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