Temporal profiling of the coding and noncoding murine cytomegalovirus transcriptomes.
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ABSTRACT:
SUBMITTER: Lacaze P
PROVIDER: S-EPMC3126304 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Publications
Temporal profiling of the coding and noncoding murine cytomegalovirus transcriptomes.
Lacaze Paul P Forster Thorsten T Ross Alan A Kerr Lorraine E LE Salvo-Chirnside Eliane E Lisnic Vanda Juranic VJ López-Campos Guillermo H GH García-Ramírez José J JJ Messerle Martin M Trgovcich Joanne J Angulo Ana A Ghazal Peter P
Journal of virology 20110406 12
The global transcriptional program of murine cytomegalovirus (MCMV), involving coding, noncoding, and antisense transcription, remains unknown. Here we report an oligonucleotide custom microarray platform capable of measuring both coding and noncoding transcription on a genome-wide scale. By profiling MCMV wild-type and immediate-early mutant strains in fibroblasts, we found rapid activation of the transcriptome by 6.5 h postinfection, with absolute dependency on ie3, but not ie1 or ie2, for gen ...[more]
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