Ontology highlight
ABSTRACT:
SUBMITTER: Kim R
PROVIDER: S-EPMC5861410 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Kim Raeyeong R Kanamaru Shuji S Mikawa Tsutomu T Prévost Chantal C Ishii Kentaro K Ito Kentaro K Uchiyama Susumu S Oda Masayuki M Iwasaki Hiroshi H Kim Seog K SK Takahashi Masayuki M
Nucleic acids research 20180301 5
Mg2+ ion stimulates the DNA strand exchange reaction catalyzed by RecA, a key step in homologous recombination. To elucidate the molecular mechanisms underlying the role of Mg2+ and the strand exchange reaction itself, we investigated the interaction of RecA with Mg2+ and sought to determine which step of the reaction is affected. Thermal stability, intrinsic fluorescence, and native mass spectrometric analyses of RecA revealed that RecA binds at least two Mg2+ ions with KD ≈ 2 mM and 5 mM. Dele ...[more]