Ontology highlight
ABSTRACT:
SUBMITTER: Acker CD
PROVIDER: S-EPMC3136788 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Acker Corey D CD Yan Ping P Loew Leslie M LM
Biophysical journal 20110701 2
We report sensitive recording of membrane potential in single dendritic spines in cortical neurons within a brain slice using two-photon excitation and a new, fluorinated, intracellularly loaded organic dye, di-2-AN(F)EPPTEA. With a two-photon excitation wavelength of 1060 nm, we achieve voltage sensitivity of >16% change in fluorescence per 100 mV. By targeting single spines in single-voxel recordings, we attain excellent single/noise quality, with back-propagating action potentials (bAPs) visi ...[more]