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Force measurements of TCR/pMHC recognition at T cell surface.

ABSTRACT: The rupture forces and adhesion frequencies of single recognition complexes between an affinity selected peptide/MHC complex and a TCR at a murine hybridoma surface were measured using Atomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends on the nature of the peptide but the rupture force does not. When CD8 is present, no effect of the nature of the peptide is observed. CD8 is proposed to act as a time and distance lock, enabling the shorter TCR molecule to bridge the pMHC and have time to finely read the peptide. Ultimately, such experiments could help the dissection of the sequential steps by which the TCR reads the peptide/MHC complex in order to control T cell activation.

SUBMITTER: Puech PH

PROVIDER: S-EPMC3142151 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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Force measurements of TCR/pMHC recognition at T cell surface.

Puech Pierre-Henri PH Nevoltris Damien D Robert Philippe P Limozin Laurent L Boyer Claude C Bongrand Pierre P

PloS one 20110722 7

The rupture forces and adhesion frequencies of single recognition complexes between an affinity selected peptide/MHC complex and a TCR at a murine hybridoma surface were measured using Atomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends on the nature of the peptide but the rupture force does not. When CD8 is present, no effect of the nature of the peptide is observed. CD8 is proposed to act as a time and distance lock, enabling the shorter TCR molecule to b ...[more]

PMID: 21799834

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