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Impact of heparan sulfate chains and sulfur-mediated bonds on the mechanical properties of bovine lens capsule.

ABSTRACT: We assessed the importance of glycosaminoglycans and sulfur-mediated bonds for the mechanical properties of lens capsules by comparing the stress-strain responses from control and treated pairs of bovine source. No significant change in mechanical properties was observed upon reduction of disulfide bonds. However, removal of glycosaminoglycan chains resulted in a significantly stiffer lens capsule, whereas high concentrations of reducing agent, which is expected to reduce the recently reported sulfilimine bond of collagen IV, resulted in a significantly less stiff lens capsule. A comparison of the diffraction patterns of the control and strongly reduced lens capsules indicated structural rearrangements on a nanometer scale.

SUBMITTER: Dyksterhuis LB

PROVIDER: S-EPMC3149272 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature

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Impact of heparan sulfate chains and sulfur-mediated bonds on the mechanical properties of bovine lens capsule.

Dyksterhuis L B LB White J F JF Hickey M M Kirby N N Mudie S S Hawley A A Vashi A A Nigro J J Werkmeister J A JA Ramshaw J A M JA

Biophysical journal 20110501 9

We assessed the importance of glycosaminoglycans and sulfur-mediated bonds for the mechanical properties of lens capsules by comparing the stress-strain responses from control and treated pairs of bovine source. No significant change in mechanical properties was observed upon reduction of disulfide bonds. However, removal of glycosaminoglycan chains resulted in a significantly stiffer lens capsule, whereas high concentrations of reducing agent, which is expected to reduce the recently reported s ...[more]

PMID: 21539774

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