Dataset Information

Synthesis and decay of varicella zoster virus transcripts.

ABSTRACT: Varicella zoster virus (VZV) is highly cell-associated. At least 68 VZV open reading frames (ORFs) are transcribed in varying amounts that increase as infection progresses. Using reverse transcriptase PCR, quantification of total and newly synthesized mRNA showed that ongoing VZV DNA replication is required for continued accumulation of VZV ORF 63, 9, and 40 transcripts. Analysis of stability of 4-thiouridine-labeled transcripts of nine VZV ORFs revealed a similar half-life for all VZV ORFs tested. Thus, difference in mRNA synthesis, and not mRNA decay, is the major factor contributing to the difference in the relative abundance of VZV transcripts in infected cells.

SUBMITTER: Azarkh Y

PROVIDER: S-EPMC3153418 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Synthesis and decay of varicella zoster virus transcripts.

Azarkh Yevgeniy Y Dölken Lars L Nagel Maria M Gilden Don D Cohrs Randall J RJ

Journal of neurovirology 20110412 3

Varicella zoster virus (VZV) is highly cell-associated. At least 68 VZV open reading frames (ORFs) are transcribed in varying amounts that increase as infection progresses. Using reverse transcriptase PCR, quantification of total and newly synthesized mRNA showed that ongoing VZV DNA replication is required for continued accumulation of VZV ORF 63, 9, and 40 transcripts. Analysis of stability of 4-thiouridine-labeled transcripts of nine VZV ORFs revealed a similar half-life for all VZV ORFs test ...[more]

PMID: 21484478

Similar Datasets

Project description:Varicella-zoster virus (VZV) is a human herpesvirus, which during primary infection typically causes varicella (chicken pox) and establishes lifelong latency in sensory and autonomic ganglia. Later in life, the virus may reactivate to cause herpes zoster (HZ; also known as shingles). To prevent these diseases, a live-attenuated heterogeneous vaccine preparation, vOka, is used routinely in many countries worldwide. Recent studies of another alphaherpes virus, infectious laryngotracheitis virus, demonstrate that live-attenuated vaccine strains can recombine in vivo, creating virulent progeny. These findings raised concerns about using attenuated herpesvirus vaccines under conditions that favor recombination. To investigate whether VZV may undergo recombination, which is a prerequisite for VZV vaccination to create such conditions, we here analyzed 115 complete VZV genomes. Our results demonstrate that recombination occurs frequently for VZV. It thus seems that VZV is fully capable of recombination if given the opportunity, which may have important implications for continued VZV vaccination. Although no interclade vaccine wild-type recombinant strains were found, intraclade recombinants were frequently detected in clade 2, which harbors the vaccine strains, suggesting that the vaccine strains have already been involved in recombination events, either in vivo or in vitro during passages in cell culture. Finally, previous partial and complete genomic studies have described strains that do not cluster phylogenetically to any of the five established clades. The additional VZV strains sequenced here, in combination with those previously published, have enabled us to formally define a novel sixth VZV clade.Although genetic recombination has been demonstrated to frequently occur for other human alphaherpesviruses, herpes simplex viruses 1 and 2, only a few ancient and isolated recent recombination events have hitherto been demonstrated for VZV. In the present study, we demonstrate that VZV also frequently undergoes genetic recombination, including strains belonging to the clade containing the vOKA strain.

Dataset Information

Synthesis and decay of varicella zoster virus transcripts.

Publications

Synthesis and decay of varicella zoster virus transcripts.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets