Ontology highlight
ABSTRACT:
SUBMITTER: Cammarata M
PROVIDER: S-EPMC3159148 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Cammarata Marco M Levantino Matteo M Schotte Friedrich F Anfinrud Philip A PA Ewald Friederike F Choi Jungkweon J Cupane Antonio A Wulff Michael M Ihee Hyotcherl H
Nature methods 20080921 10
We demonstrate tracking of protein structural changes with time-resolved wide-angle X-ray scattering (TR-WAXS) with nanosecond time resolution. We investigated the tertiary and quaternary conformational changes of human hemoglobin under nearly physiological conditions triggered by laser-induced ligand photolysis. We also report data on optically induced tertiary relaxations of myoglobin and refolding of cytochrome c to illustrate the wide applicability of the technique. By providing insights int ...[more]