Microfluidic-based 18F-labeling of biomolecules for immuno-positron emission tomography.
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ABSTRACT:
SUBMITTER: Liu K
PROVIDER: S-EPMC3163899 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Publications
Microfluidic-based 18F-labeling of biomolecules for immuno-positron emission tomography.
Liu Kan K Lepin Eric J EJ Wang Ming-Wei MW Guo Feng F Lin Wei-Yu WY Chen Yi-Chun YC Sirk Shannon J SJ Olma Sebastian S Phelps Michael E ME Zhao Xing-Zhong XZ Tseng Hsian-Rong HR Michael van Dam R R Wu Anna M AM Shen Clifton K-F CK
Molecular imaging 20110601 3
Methods for tagging biomolecules with fluorine 18 as immuno-positron emission tomography (immunoPET) tracers require tedious optimization of radiolabeling conditions and can consume large amounts of scarce biomolecules. We describe an improved method using a digital microfluidic droplet generation (DMDG) chip, which provides computer-controlled metering and mixing of 18F tag, biomolecule, and buffer in defined ratios, allowing rapid scouting of reaction conditions in nanoliter volumes. The ident ...[more]
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