Ontology highlight
ABSTRACT:
SUBMITTER: Coburn GA
PROVIDER: S-EPMC317069 | biostudies-literature | 1999 Oct
REPOSITORIES: biostudies-literature
Coburn G A GA Miao X X Briant D J DJ Mackie G A GA
Genes & development 19991001 19
The RNA degradosome is a multiprotein complex required for the degradation of highly structured RNAs. We have developed a method for reconstituting a minimal degradosome from purified proteins. Our results demonstrate that a degradosome-like complex containing RNase E, PNPase, and RhlB can form spontaneously in vitro in the absence of all other cellular components. Moreover, ATP-dependent degradation of the malEF REP RNA by the reconstituted, minimal degradosome is indistinguishable from that of ...[more]